Additional Data File 1 Homophilic Interaction of Unc-69
نویسنده
چکیده
unc-69(ok339) deletion unc-69(ok339) deletes a 2.65 kb genomic fragment encompassing the whole unc-69 transcription unit as well as flanking sequences both 5’ and 3’ of the gene (Supplemental Figure S1). Thus, this deletion is certain to represent a null allele of unc-69. unc-69(ok339) homozygotes have an Unc phenotype and arrest during L1 to L2 transition (Supp. Table S2). We found that ok339 also deletes T07A5.5 (predicted to encode the C. elegans homolog of the Ost4p subunit of the S. cerevisiae oligosaccharyltransferase) and ends but 200 bp 5’ of the unc-50 coding region (unpublished). We reasoned that the ok339 arrest phenotype could either be due to loss of T07A5.5, or a synthetic phenotype due to the simultaneous loss of both unc-50 and unc-69 function. Indeed, unc-69(ok339) mutant worms were resistant to 25mM levamisole, a hallmark of unc-50 mutations. Furthermore the ok339 deletion failed to complement unc50(e306) mutants (data not shown). We balanced unc69(ok339) with qC1, and microinjected a 6.7 kb Hind III-EcoR I genomic fragment (pUnc50-10) carrying wild-type copies of both unc-50 and T07A5.5 into the deletion carrying strain. Transgenic worms homozygous for unc69(ok339) grew to adulthood but were sterile (three independent lines). It is likely that the sterility is due to lack of germline expression of T07A5.5 off the extrachromosomal array. For this reason, we did not pursue usage of unc-69(ok339) in our studies.
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